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KMID : 1149220090170010175
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Korean Journal of Oriental Medical Prescription
2009 Volume.17 No. 1 p.175 ~ p.185
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Anti-inflammtory effects of the MeOH extract of Petiolus Nelumbinis
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Lee Won-Wook
Jo Mi-Jeong
Park Sang-Mi
Jung Ji-Yun
Kim Sang-Chan
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Abstract
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Petiolus Nelumbinis, branches of lotus leaf or lotus flower is a traditional oriental herbal medicine widely used for treating a superheat or disorder of qi flow. Although there are many clinical results and literature study, it has been rarely conducted to evaluate the immuno-biological activity. The present study was conducted to examine the anti-inflammatory effects of PNM (Petiolus Nelumbinis MeOH extract) in vitro. To determine cytotoxic concentration of PNM, the cells were treated with PNM for 24 h after LPS addition, and the cell viability was tested by MTT assay. Both of dosages (30 and 100 μg/ml) of PNM had no cytotoxicity. In these concentrations, PNM significantly reduced the elevated levels of NO and PGE2 by LPS. These inhibitory effects of PNM were due to the reduced expressions of iNOS and COX-2 protein. TNF-α, IL-1β and IL-6 are frequently encountered pro-inflammatory cytokines, and LPS plays a key role in inducing to the massive production of these cytokines. Thus, we next determined the levels of these cytokines. Although PNM had no significant inhibitory effect on the production of TNF-α, the elevated levels of IL-1β and IL-6 by LPS were dose-dependently reduced in PNM-treated groups. These results demonstrate that PNM has anti-inflammatory effects by inhibiting the production of proinflammatory cytokines, NO and PGE2 in LPS-activated macrophage. Moreover, the reduction of NO and PGE2 levels was due to the inhibition of iNOS and COX-2 protein expression by PNM.
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KEYWORD
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Petiolus Nelumbinis, Anti-inflammatory effects, LPS, Cytokine
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